DNA ligases seal five′-PO4 and three′-OH polynucleotide ends through 3 nucleotidyl transfer methods involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are crucial guardians of genomic integrity, and ligase dysfunction underlies human genetic condition syndromes. Crystal structures of DNA ligases sure to nucleotide and nucleic acid substrates have illuminated how ligase reaction chemistry is catalyzed, how ligases acknowledge destroyed DNA ends, and how protein area actions and active-site transforming are used to choreograph the tip-joining pathway.
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